Doxorubicin Induced Histomorphometric Changes in the Kidney of Albino Rats and Protective Role of Nigella Sativa


Wazir Ahmed Baloch, Shahid Zafar, Ihsan Ullah, Muhammad Imran Khan, Maher Sohail Yaseen and Muhammad Kamran Ameer

Objective: Doxorubicin is presently a leading antineoplastic drug with promising efficacy. This study was designed to investigate the histological
effects of doxorubicin toxicity on rat kidneys and how much protection is provided by Nigella Sativa.
Materials & Methods: A randomized controlled trial conducted on thirty adult male wistar rats divided randomly into three equal groups. Group A served as a control. Group B was injected with weekly intraperitoneal injections of doxorubicin at a dose of 3mg/kg b.w. Group C rats received doxorubicin along with nigella sativa at a dose of 1000mg/kg b.w. orally
daily. At the end of these interventions, animals were sacrificed, and kidneys were removed for the purpose of histological staining. Renal glomerular and tubules related histopathological parameters were assessed
qualitatively as mild, moderate & severe. Renal glomerular diameter was digitally measured by microscope. Ethical approval was taken from Ethical
Committee, Jinnah Postgraduate Medical Centre (JPMC), Karachi. All the parameters were statistically analyzed.
Results: Group B rats’ renal tissue was adversely affected by the drug showing marked necrosis of tubules and the glomeruli along with interstitial cells’ infiltration. Glomerular diameter was also significantly decreased in group B as compared to group A. These histological features in group C rats’ renal tissue were milder and glomerular diameter was close to that of group A.
Conclusion: According to our study doxorubicin treatment proved noxious for the renal tissue, both tubules and glomeruli, while nigella sativa significantly competed against these pathological alterations.

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